1. The Plant Variety Protection (PVP) Authority decides when, where and in what quantity and quality the seed material required for testing the variety is to be delivered. Applicants submitting material from a country other than India must make sure that all customs formalities are complied with. The minimum quantity of seed to be supplied by the applicant should be :

Variety and hybrid - 3000 grams (in only one submission)

Parental lines - 1500 grams

2. The seed should meet the minimum requirements for germination capacity, moisture content and physical purity prescribed for certified seed in India . Especially for storage, which requires a higher standard, the applicant should state, the actual germination capacity which should be as high as possible.

3 The seed material must not have undergone any treatment unless the competent authorities allow or request such treatment. If it has been treated, full details of the treatment must be given.

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III. Conduct of tests

1. The minimum duration of tests should normally be two independent similar growing seasons with reference to the ecosystem of the variety submitted for DUS test.

2. The test should normally be conducted at two test locations. If any important characteristics of the variety can not be seen at these places, the variety may be tested at an additional place.

3. The field tests should be carried out under conditions ensuring normal growth. The size of the plots should be such that plants or parts of plants may be removed for measurement and counting without prejudice to the observations which must be made upto the end of the growing period. Each test should include about 400 plants which should be divided between two replications. Separate plots for observation and for measurement can only be used if they have been subjected to similar environmental conditions.

4. Test plot design

Number of rows	: 8
Row length	: 5 m
Row to row distance	: 45 cm
Plant to plant distance	: 20 cm
Number of replications	: 2

5. Observations should not be recorded on plants in border rows.

6. Additional tests for special purpose may be established.

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IV. Methods and observations

The characteristics described in the table of characteristics should be used for the testing of varieties, inbred lines and hybrids for DUS.

For the assessment of distinctness and stability observations should be made on 20 plants or parts of 20 plants which should be divided between 2 replications (10 plants per replication).

For the assessment of uniformity of characteristics on the plot as a whole (visual assessment by a single observation of a group of plants or parts of plants), the number of aberrant plants or parts of plants should not exceed 5%

For the assessment of uniformity of characteristics on single plants or parts of plants (visual assessment by observations of a number of individual plants or parts of plants), the number of aberrant plants or parts of plants should not exceed 5%.

All observations on the first leaf should be made at the 6 leaves stage.

All observations on the plant, the sixth leaf and the bract should be made at the time of flowering.

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V. Grouping of varieties

1. The collection to be grown should be divided into groups to facilitate the assessment of distinctness. Characteristics that are suitable for grouping purposes are those which are known from experience not to vary, or to vary only slightly, within a variety and which in their various states are fairly evenly distributed within the collection.

2. It is recommended that the competent authorities use the following characteristics for grouping varieties.

i. Time of flowering (Characteristic 7)

ii. Petal : color (Characteristic 10)

iii. Petal : change of color (faded stage) (Characteristic 11)

iv. Plant : height (Characteristic 23)

v. Seed : oil content (Characteristic 29)

vi. Seed : percentage of oleic acid (Characteristic 30)

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VI. Characteristics and symbols

1. To assess distinctness, uniformity and stability, the characteristics and their states as given in the Table of Characteristics should be used.

2. Notes (1 to 9), for the purposes of electronic data processing, are given opposite the states of the different characteristics.

3. Legend :

(*) Characteristics that should be used every growing period for the examinations of all varieties and should always be included in the description of the variety, except when the state of expression of a preceding characteristic or regional environmental conditions render this impossible.

(+) See Explanations on the table of characteristics in Chapter VIII.

4. The optimum stage of plant growth for assessment of each characteristics is indicated in the sixth column of table of characteristics.

5. Type of assessment of characteristics indicated in column 7 of table of characteristics is as follows :

MG : Measurement by a single observation of a group of plants or parts of plants

MS : Measurement of a number of individual plants or parts of plants

VG : Visual assessment by a single observation of a group of plants or parts of plants

VS : Visual assessment by observations of individual plants or parts of plants

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VII. Table of characteristics

S.No.	Characteristics	State	Note	Example varieties	Stage of observation	Type of assessment
1.	First leaf : length of blade	very short short medium long very long	1 3 5 7 9		sixth leaf stage	MS
2.	First leaf : width of blade	very narrow narrow medium broad very broad	1 3 5 7 9		sixth leaf stage	MS
3.	First leaf : ratio length/width of blade	very low low medium high very high	1 3 5 7 9		sixth leaf stage	MS
4.	First leaf : length of petiole	absent medium long	1 5 9		sixth leaf stage	VG
5.	First leaf : number of spines	absent few medium many very many	1 3 5 7 9		sixth leaf stage	VG
6. (+)	First leaf : Dentations	absent or very weak weak medium strong very strong	1 3 5 7 9		sixth leaf stage	VG
7. (*)	Time of flowering (50% of plants with atleast one inflorescence open)	very early (>51 days) early (51-70 days) medium (71-80 days) late (81-90 days) very late (>91 days)	1 3 5 7 9		50% of plants with atleast one inflorescence open	VG
8.	Plant : height of insertion of first branch (from ground level)	very short (<6 cm) short (6-15 cm) medium (16-25 cm) tall (26-35 cm) very tall (>35 cm)	1 3 5 7 9		flowering	MS
9.	Plant : length of longest primary branch	very short short medium long very long	1 3 5 7 9		flowering	MS
10. (*)	Petal : color	white yellow pink orange mixed	1 2 3 4 5	JSF-1 Bhima A-1, Manjira	flowering	VG
11. (*)	Petal : change of colour (faded stage)	grey white pinkish white golden yellow orange red	1 2 3 4 5	JSF-1 Bhima APRR-3 Manjira HUS-305	10 days after flowering	VG
12.	Sixth leaf : length of blade	very short short medium long very long	1 3 5 7 9		flowering	MS
13.	Sixth leaf : width of blade	very narrow narrow medium broad very broad	1 3 5 7 9		flowering	MS
15.	Sixth leaf : ratio length/ width of blade	very low low medium high very high	1 3 5 7 9		flowering	MS
15. (+)	Sixth leaf : shape	fusiform ovate elliptic obovate	1 2 3 4		flowering	VG
16.	Sixth leaf : number of spines	absent few medium many very many	1 3 5 7 9		flowering	VG
17.	Sixth leaf : Dentations	absent weak medium strong very strong	1 3 5 7 9		flowering	VG
18.	Capitulum : length of outer involucral bract of main capitula	short medium long	3 5 7		flowering	MS
19.	Capitulum : width of outer involucral bract of main capitula	narrow medium broad	3 5 7		flowering	MS
20.	Capitulum : ratio of length/width of outer involucral bract of main capitula	low medium high	3 5 7		flowering	MS
21	Capitulum : number of spines of outer involucral bract of main capitula	absent few medium many very many	1 3 5 7 9		flowering	MS
22.	Capitulum : diameter of main capitula	small (<2.0 cm) medium (2.0-2.5 cm) large (>2.5 cm)	3 5 7		dough grain	MS
23. (*)	Plant : height upto main capitula	very short (<51 cm) short (51-60 cm) medium (61-70 cm) tall (71-80 cm) very tall (>80 cm)	1 3 5 7 9		harvest maturity	MS
24	Seed : 1,000 seed weight	very low (<41 g) low (41-50 g) medium (51-60 g) high (61-70 g) very high (>70 g)	1 3 5 7 9		harvest maturity	MG
25	Seed : size	small medium large	3 5 7		harvest maturity	VG
26.	Seed : colour	white white yellowish brown yellowish brown	1 2 3 4		harvest maturity	VG
27.	Seed : number/ main capitula	low (<15) medium (15-30) high (>30)	3 5 7		harvest maturity	MS
28.	Hull content	low medium high	3 5 7		harvest maturity	MG
29. (*)	Seed : oil content	low (25%) medium (25-30%) high (>30%)	3 5 7		harvest maturity	MG
30. (*)	Seed : percentage of oleic acid	low (<10) medium (10-25) high (>25)	3 5 7		harvest maturity	MG
31. (*)	Seed : percentage of linoleic acid	low (<65) medium (65-75) high (>75)	3 5 7		harvest maturity	MG

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VIII. Explanations on the table of characteristics

Characteristic 6. First leaf : dentation

Characteristic 15. Sixth leaf : shape

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IX. Technical questionnaire

Reference Number
(not to be filled in by the applicant)

Technical questionnaire
to be completed in connection with an application for plant breeders' rights

1. Species: Carthamus tinctorius L.
Safflower

2. Applicant (Name and address) :

3. Proposed denomination or breeder's reference

4. Information on origin, maintenance and reproduction of the variety

4.1 Type of material

(i) inbred line	( )
(ii) single cross hybrid	( )
(iii) three-way cross hybrid	( )
(iv) double cross hybrid	( )
(v) open-pollinated variety	( )
(vi) other (indicate formula)	( )

4.2 Formula (hybrid only)

4.3 Genetic origin and breeding method (source, crossing, mutation etc.)

4.4 Maintenance

4.5 Use

5. Characteristics of the variety to be indicated (the number in brackets refers to the corresponding characteristic in Test Guidelines; please mark the state of expression which best corresponds).

S.No.

Characteristics

States

Example varieties

Notes

5.1
(7)

Time of flowering (50% of plants with at least one inflorescence open

very early (>51 days)
early (51-70 days)
medium (71-80 days)
late (81-90 days)
very late (>91 days

1 [ ]
3 [ ]
5 [ ]
7 [ ]
9 [ ]

5.2
(10)

Petal : colour

white
yellow
pink
orange
mixed

1 [ ]
2 [ ]
3 [ ]
4 [ ]
5 [ ]

5.3
(11)

Petal : change of colour (faded stage)

grey white
pinkish white
golden yellow
orange
red

1 [ ]
2 [ ]
3 [ ]
4 [ ]
5 [ ]

5.4.
(23)

Plant : height

very short (<51 cm)
short (51-60 cm)
medium (61-70 cm)
tall (71-80 cm)
very tall (>80 cm)

1 [ ]
3 [ ]
5 [ ]
7 [ ]
9 [ ]

5.5.
(29)

Seed : oil content

low
medium
high

3 [ ]
5 [ ]
7 [ ]

5.6
(30)

Seed : percentage of oleic acid

low
medium
high

3 [ ]
5 [ ]
7 [ ]

6. Similar varieties and differences from these varieties

Denomination of
Similar variety

Characteristic in
which the similar variety is different ⁰⁾

State of expression of similar Variety

State of expression of candidate variety

⁰⁾In the case of identical states of expressions of both varieties, please indicate the size of the difference.

7. Additional information which may help to distinguish the variety

7.1 Resistance to pests and diseases

7.2 Special conditions for the examination of the variety

7.3 Other information

8. Authorization for release

(a) Does the variety require prior authorization for release under legislation concerning the protection of the environment, human and animal health?

Yes { } No { }

(b) Has such authorization been obtained?

Yes { } No { }

If the answer to that question is ‘Yes’, please attach a copy of such an authorization

[End of the document]

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